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Becton Dickinson
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Addgene inc
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Santa Cruz Biotechnology
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Image Search Results
Journal: Molecular Brain
Article Title: Mesenchymal stem cells suppress neuronal apoptosis and decrease IL-10 release via the TLR2/NFκB pathway in rats with hypoxic-ischemic brain damage
doi: 10.1186/s13041-015-0157-3
Figure Lengend Snippet: Pam3CSK4 exacerbated learning-memory dysfunction and stimulated the TLR2/NFκB signaling pathway and IL-10 secretion in HIBD rats. a Diagram illustrating the experimental protocols of treatments and tests used in the rats. b - c The escape latencies and path lengths to reach the visible platform on the first day of the Morris water maze test for the HIBD and Pam3CSK4 groups. d The escape latencies to reach the visible platform on the 2 nd through the 5 th days of the Morris water maze test for the Pam3CSK4-treated and HIBD groups. e The duration spent in the hidden platform quadrant by the Pam3CSK4-treated and HIBD groups. n = 20 in each group. f - g The Pam3CSK4-induced mRNA and protein expression levels of TLR2, NFκB P65 and Bax in the brains of 4-week-old rats. n = 5 in each group. h The quantifications of WB signal in G. i IL-10 release in the brains of 4-week-old rats in HIBD and Pam3CSK4 groups. n = 6 in each group. # P < 0.05 vs. the HIBD group
Article Snippet: The anti-TLR2 (1:1000, Abcam, ab13855),
Techniques: Expressing
Journal: Molecular Brain
Article Title: Mesenchymal stem cells suppress neuronal apoptosis and decrease IL-10 release via the TLR2/NFκB pathway in rats with hypoxic-ischemic brain damage
doi: 10.1186/s13041-015-0157-3
Figure Lengend Snippet: Co-culturing OGD-injured PC12 cells with MSCs decreased the expression of TLR2, NFκB, Bax and IL-10. a - b The mRNA and protein expression levels of TLR2 in OGD-injured PC12 cells in the presence or absence of MSCs. n = 6 in each group. c The quantifications of WB signal in b . d - e The mRNA and protein expression levels of NFκB P65 in OGD-injured PC12 cells in the presence or absence of MSCs. n = 6 in each group. f The quantifications of WB signal in e . g - h The mRNA and protein expression levels of Bax in OGD-injured PC12 cells in the presence or absence of MSCs. n = 6 in each group. i The quantifications of WB signal in h . j The concentrations of IL-10 released into the culture media were measured in the control, OGD and OGD + MSCs groups via ELISA. n = 6 in each group. # P < 0.05 vs. the control PC12 cells; & P < 0.05 vs. the OGD-injured PC12 cells
Article Snippet: The anti-TLR2 (1:1000, Abcam, ab13855),
Techniques: Expressing, Control, Enzyme-linked Immunosorbent Assay
Journal: Molecular Brain
Article Title: Mesenchymal stem cells suppress neuronal apoptosis and decrease IL-10 release via the TLR2/NFκB pathway in rats with hypoxic-ischemic brain damage
doi: 10.1186/s13041-015-0157-3
Figure Lengend Snippet: MSCs modulated the TLR2/NFκB signaling pathway and suppressed the apoptosis of OGD-injured PC12 cells and the secretion of IL-10. a Diagram illustrating the experimental protocols of the treatments and tests for PC12 cells. b - c The TLR2 mRNA and protein expression levels in co-cultures of MSCs with OGD-injured PC12 cells that received Pam3CSK4 or Ad-siTLR2 treatment. n = 6 in each group. d The quantifications of WB signal in c . e - f The mRNA and protein expression levels of NFκB P65 in co-cultures of MSCs with OGD-injured PC12 cells that were treated with Pam3CSK4 or Ad-siTLR2. n = 6 in each group. g The quantifications of WB signal in f . h - i The changes in the mRNA and protein expression levels of Bax in co-cultures of MSCs with OGD-injured PC12 cells that were treated with Pam3CSK4 or Ad-siTLR2. n = 6 in each group. j The quantifications of WB signal in i . k The changes in IL-10 release in co-cultures of MSCs with OGD-injured PC12 cells that received Pam3CSK4 or Ad-siTLR2 treatment. n = 6 in each group. # P < 0.05 vs. OGD-injured PC12 cells that were treated with Pam3CSK4 or Ad-siTLR2
Article Snippet: The anti-TLR2 (1:1000, Abcam, ab13855),
Techniques: Expressing
Journal: Molecular Brain
Article Title: Mesenchymal stem cells suppress neuronal apoptosis and decrease IL-10 release via the TLR2/NFκB pathway in rats with hypoxic-ischemic brain damage
doi: 10.1186/s13041-015-0157-3
Figure Lengend Snippet: Primers of the target genes used for real-time PCR
Article Snippet: The anti-TLR2 (1:1000, Abcam, ab13855),
Techniques: